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Stimulation by LPS and HMGB1 in peripheral blood neutrophils from patients with sepsis-induced acute lung injury-GSE3037

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Clinical

Stimulation by LPS and HMGB1 in peripheral blood neutrophils from patients with sepsis-induced acute lung injury-GSE3037

Purpose

Peripheral blood neutrophils were isolated from septic patients and treated in vitro with LPS or HMGB1

Hypothesis

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Experimental Design

8 patients, each contributing a control, LPS-treated, and HMGB1-treated neutrophil sample.

Experimental Variables

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Controls

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Methods

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Additional Information
  • Silva E, Arcaroli J, He Q, Svetkauskaite D et al. HMGB1 and LPS induce distinct patterns of gene expression and activation in neutrophils from patients with sepsis-induced acute lung injury. Intensive Care Med 2007 Oct;33(10):1829-39. PMID: 17581740
    • Microarray
    Affymetrix HG-U133A
    24 Samples Loaded: 24
    Human (Homo sapiens)
    Neutrophils
    Sepsis
    Sample Set Spreadsheet
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    Samples Preview
    Sample ID !Sample_title none provided Culture conditions
    GSM66867 Patient 05 control True CTRL
    GSM66868 Patient 06 control True CTRL
    GSM66869 Patient 07 control True CTRL
    GSM66870 Patient 08 control True CTRL
    GSM66871 Patient 09 control True CTRL

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    File
    sampleset4000029_sampleannotations.csv
    Sample Set Spreadsheet
    Raw Signal
    Raw Signal
    •   |  View in GXB »
    Clinical Datasource
    Links
    http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE3037
    http://www.ncbi.nlm.nih.gov/pubmed/17581740

    Samples Viewer / Editor

    All fields are editable except the "Sample ID" column. To edit a cell, click within the cell. To edit a "date" cell, click on the calendar icon. To cancel an edit, press the ESC key.

    Sample ID !Sample Title None provided Culture conditions
    GSM66867
    Patient 05 control
    True
    CTRL
    GSM66868
    Patient 06 control
    True
    CTRL
    GSM66869
    Patient 07 control
    True
    CTRL
    GSM66870
    Patient 08 control
    True
    CTRL
    GSM66871
    Patient 09 control
    True
    CTRL
    GSM66872
    Patient 12 control
    True
    CTRL
    GSM66873
    Patient 15 control
    True
    CTRL
    GSM66874
    Patient 19 control
    True
    CTRL
    GSM66875
    Patient 05 HMGB1 treated
    True
    HMGB
    GSM66876
    Patient 06 HMGB1 treated
    True
    HMGB
    GSM66877
    Patient 07 HMGB1 treated
    True
    HMGB
    GSM66878
    Patient 08 HMGB1 treated
    True
    HMGB
    GSM66879
    Patient 09 HMGB1 treated
    True
    HMGB
    GSM66880
    Patient 12 HMGB1 treated
    True
    HMGB
    GSM66881
    Patient 15 HMGB1 treated
    True
    HMGB
    GSM66882
    Patient 19 HMGB1 treated
    True
    HMGB
    GSM66883
    Patient 05 LPS treated
    True
    LPS
    GSM66884
    Patient 06 LPS treated
    True
    LPS
    GSM66885
    Patient 07 LPS treated
    True
    LPS
    GSM66886
    Patient 08 LPS treated
    True
    LPS
    GSM66887
    Patient 09 LPS treated
    True
    LPS
    GSM66888
    Patient 12 LPS treated
    True
    LPS
    GSM66889
    Patient 15 LPS treated
    True
    LPS
    GSM66890
    Patient 19 LPS treated
    True
    LPS

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