Sample Set Annotation Tool: PGN induced transcriptional changes in human neonatal neutrophils-GSE40636

Study Design
Assay
Sample Info
Files
Info
Samples
Group Sets
Analysis

Purpose

We have employed whole genome microarray expression profiling to identify genes differentially expressed in cord blood purified neutrophils after a short-term exposure to peptidoglycan (PGN).

Hypothesis

Experimental Design

PGN induced gene expressions of neonatal neutrophils were measured at 4 hours. Three biological replicates were performed for each treatment group.

Experimental Variables

Controls

Methods

Additional Information

Fong ON, Chan KY, Leung KT, Lam HS et al. Expression profile of cord blood neutrophils and dysregulation of HSPA1A and OLR1 upon challenge by bacterial peptidoglycan. J Leukoc Biol 2014 Jan;95(1):169-78. PMID: 23986550

Expected Samples:

6 Samples Loaded: 6

Species

Human (Homo sapiens)

Sample Source

Neutrophils

Disease

In vitro stimulation

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	cell type	treatment	source tissue
GSM998570	CB1 CTL, biological replicate 1	neonatal neutrophil	control	cord blood
GSM998571	CB1-PGN, biological replicate 1	neonatal neutrophil	peptidoglycan	cord blood
GSM998572	CB2 CTL, biological replicate 2	neonatal neutrophil	control	cord blood
GSM998573	CB2-PGN, biological replicate 2	neonatal neutrophil	peptidoglycan	cord blood
GSM998574	CB3 CTL, biological replicate 3	neonatal neutrophil	control	cord blood

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File

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Sample ID	!Sample Title	Cell type	Treatment	Source tissue
GSM998570	CB1 CTL, biological replicate 1	neonatal neutrophil	control	cord blood
GSM998571	CB1-PGN, biological replicate 1	neonatal neutrophil	peptidoglycan	cord blood
GSM998572	CB2 CTL, biological replicate 2	neonatal neutrophil	control	cord blood
GSM998573	CB2-PGN, biological replicate 2	neonatal neutrophil	peptidoglycan	cord blood
GSM998574	CB3 CTL, biological replicate 3	neonatal neutrophil	control	cord blood
GSM998575	CB3-PGN, biological replicate 3	neonatal neutrophil	peptidoglycan	cord blood

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Module Analysis

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SIDRA