Sample Set Annotation Tool: Expression changes induced by transmigration in human neutrophils-GSE2322

Study Design
Assay
Sample Info
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Info
Samples
Group Sets
Analysis

Purpose

Human volunteers were exposed to endotoxin by bronchoscopic instillation, airspace and circulating neutrophils were isolated 16 hours later and compared to circulating neutrophils obtained prior to endotoxin exposure, and to circulating neutrophils exposed to endotoxin in vitro.

Hypothesis

Experimental Design

Experimental Variables

Controls

Methods

Additional Information

Coldren CD, Nick JA, Poch KR, Woolum MD et al. Functional and genomic changes induced by alveolar transmigration in human neutrophils. Am J Physiol Lung Cell Mol Physiol 2006 Dec;291(6):L1267-76. PMID: 16861384

Expected Samples:

58 Samples Loaded: 58

Species

Human (Homo sapiens)

Sample Source

Neutrophils

Disease

Healthy

Treatment Protocol

Growth Protocol

Extraction Protocol

Storage Conditions

Sample Set Spreadsheet

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Samples Preview

Sample ID	!Sample_title	volunteer 89 Post-Endotoxin Instillation Circulating Neutrophils	volunteer 90 Airspace Neutrophils	volunteer 21 Pre-Endotoxin Instillation Circulating Neutrophils	volunteer 59 Post-Endotoxin Instillation Circulating Neutrophils
GSM42999	volunteer 09 Airspace Neutrophils
GSM43000	volunteer 09 Post-Endotoxin Instillation Circulating Neutrophils
GSM43001	volunteer 16 Airspace Neutrophils
GSM43002	volunteer 16 Post-Endotoxin Instillation Circulating Neutrophils
GSM43003	volunteer 21 Airspace Neutrophils

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File
sampleset4000027_sampleannotations.csv Sample Set Spreadsheet

Samples Viewer / Editor

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Sample ID	!Sample Title	Volunteer 89 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 90 Airspace Neutrophils	Volunteer 21 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 59 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 84 Airspace Neutrophils	Volunteer 59 Airspace Neutrophils	Volunteer 90 in vitro Endotoxin Neutrophils	Volunteer 87 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 90 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 60 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 37 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 84 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 53 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 88 in vitro Control Neutrophils	Volunteer 88 in vitro Endotoxin Neutrophils	Volunteer 90 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 88 Airspace Neutrophils	Volunteer 75 in vitro Control Neutrophils	Volunteer 84 in vitro Endotoxin Neutrophils	Volunteer 89 Airspace Neutrophils	Volunteer 90 in vitro Control Neutrophils	Volunteer 70 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 70 Airspace Neutrophils	Volunteer 80 Airspace Neutrophils	Volunteer 48 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 75 in vitro Endotoxin Neutrophils	Volunteer 80 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 87 Airspace Neutrophils	Volunteer 49 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 32 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 37 Airspace Neutrophils	Volunteer 84 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 21 Airspace Neutrophils	Volunteer 59 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 49 Airspace Neutrophils	Volunteer 75 Airspace Neutrophils	Volunteer 49 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 21 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 75 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 88 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 16 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 60 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 48 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 87 in vitro Control Neutrophils	Volunteer 88 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 32 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 16 Airspace Neutrophils	Volunteer 53 Airspace Neutrophils	Volunteer 84 in vitro Control Neutrophils	Volunteer 32 Airspace Neutrophils	Volunteer 70 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 89 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 48 Airspace Neutrophils	Volunteer 09 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 37 Post-Endotoxin Instillation Circulating Neutrophils	Volunteer 09 Airspace Neutrophils	Volunteer 53 Pre-Endotoxin Instillation Circulating Neutrophils	Volunteer 87 in vitro Endotoxin Neutrophils	Conditions
GSM42999	volunteer 09 Airspace Neutrophils																																																								True			Air_PMN
GSM43000	volunteer 09 Post-Endotoxin Instillation Circulating Neutrophils																																																						True					POST
GSM43001	volunteer 16 Airspace Neutrophils																																															True												Air_PMN
GSM43002	volunteer 16 Post-Endotoxin Instillation Circulating Neutrophils																																									True																		POST
GSM43003	volunteer 21 Airspace Neutrophils																																	True																										Air_PMN
GSM43004	volunteer 21 Post-Endotoxin Instillation Circulating Neutrophils																																						True																					POST
GSM43005	volunteer 21 Pre-Endotoxin Instillation Circulating Neutrophils			True																																																								PRE
GSM43006	volunteer 32 Airspace Neutrophils																																																		True									Air_PMN
GSM43007	volunteer 32 Post-Endotoxin Instillation Circulating Neutrophils																														True																													POST
GSM43008	volunteer 32 Pre-Endotoxin Instillation Circulating Neutrophils																																														True													PRE
GSM43009	volunteer 37 Airspace Neutrophils																															True																												Air_PMN
GSM43010	volunteer 37 Post-Endotoxin Instillation Circulating Neutrophils																																																							True				POST
GSM43011	volunteer 37 Pre-Endotoxin Instillation Circulating Neutrophils											True																																																PRE
GSM43012	volunteer 48 Airspace Neutrophils																																																					True						Air_PMN
GSM78522	volunteer 48 Post-Endotoxin Instillation Circulating Neutrophils																																											True																POST
GSM78523	volunteer 48 Pre-Endotoxin Instillation Circulating Neutrophils																									True																																		PRE
GSM78524	volunteer 49 Airspace Neutrophils																																			True																								Air_PMN
GSM78525	volunteer 49 Post-Endotoxin Instillation Circulating Neutrophils																													True																														POST
GSM78526	volunteer 49 Pre-Endotoxin Instillation Circulating Neutrophils																																					True																						PRE
GSM78527	volunteer 53 Airspace Neutrophils																																																True											Air_PMN
GSM78528	volunteer 53 Post-Endotoxin Instillation Circulating Neutrophils													True																																														POST
GSM78529	volunteer 53 Pre-Endotoxin Instillation Circulating Neutrophils																																																									True		PRE
GSM78530	volunteer 59 Airspace Neutrophils						True																																																					Air_PMN
GSM78531	volunteer 59 Post-Endotoxin Instillation Circulating Neutrophils				True																																																							POST
GSM78532	volunteer 59 Pre-Endotoxin Instillation Circulating Neutrophils																																		True																									PRE
GSM78533	volunteer 60 Post-Endotoxin Instillation Circulating Neutrophils																																										True																	POST
GSM78534	volunteer 60 Pre-Endotoxin Instillation Circulating Neutrophils										True																																																	PRE
GSM78535	volunteer 70 Airspace Neutrophils																							True																																				Air_PMN
GSM78536	volunteer 70 Post-Endotoxin Instillation Circulating Neutrophils																																																			True								POST
GSM78537	volunteer 70 Pre-Endotoxin Instillation Circulating Neutrophils																						True																																					PRE
GSM78538	volunteer 75 Airspace Neutrophils																																				True																							Air_PMN
GSM78539	volunteer 75 in vitro Control Neutrophils																		True																																									PMN_CTRL
GSM78540	volunteer 75 in vitro Endotoxin Neutrophils																										True																																	PMN_Toxin
GSM78541	volunteer 75 Post-Endotoxin Instillation Circulating Neutrophils																																							True																				POST
GSM78542	volunteer 80 Airspace Neutrophils																								True																																			Air_PMN
GSM78543	volunteer 80 Pre-Endotoxin Instillation Circulating Neutrophils																											True																																PRE
GSM78544	volunteer 84 Airspace Neutrophils					True																																																						Air_PMN
GSM78545	volunteer 84 in vitro Control Neutrophils																																																	True										PMN_CTRL
GSM78546	volunteer 84 in vitro Endotoxin Neutrophils																			True																																								PMN_Toxin
GSM78547	volunteer 84 Post-Endotoxin Instillation Circulating Neutrophils												True																																															POST
GSM78548	volunteer 84 Pre-Endotoxin Instillation Circulating Neutrophils																																True																											PRE
GSM78549	volunteer 87 Airspace Neutrophils																												True																															Air_PMN
GSM78550	volunteer 87 in vitro Control Neutrophils																																												True															PMN_CTRL
GSM78551	volunteer 87 in vitro Endotoxin Neutrophils																																																										True	PMN_Toxin
GSM78552	volunteer 87 Post-Endotoxin Instillation Circulating Neutrophils								True																																																			POST
GSM78553	volunteer 88 Airspace Neutrophils																	True																																										Air_PMN
GSM78554	volunteer 88 in vitro Control Neutrophils														True																																													PMN_CTRL
GSM78555	volunteer 88 in vitro Endotoxin Neutrophils															True																																												PMN_Toxin
GSM78556	volunteer 88 Post-Endotoxin Instillation Circulating Neutrophils																																								True																			POST
GSM78557	volunteer 88 Pre-Endotoxin Instillation Circulating Neutrophils																																													True														PRE
GSM78558	volunteer 89 Airspace Neutrophils																				True																																							Air_PMN
GSM78559	volunteer 89 Post-Endotoxin Instillation Circulating Neutrophils	True																																																										POST
GSM78560	volunteer 89 Pre-Endotoxin Instillation Circulating Neutrophils																																																				True							PRE
GSM78561	volunteer 90 Airspace Neutrophils		True																																																									Air_PMN
GSM78562	volunteer 90 in vitro Control Neutrophils																					True																																						PMN_CTRL
GSM78563	volunteer 90 in vitro Endotoxin Neutrophils							True																																																				PMN_Toxin
GSM78564	volunteer 90 Post-Endotoxin Instillation Circulating Neutrophils																True																																											POST
GSM78565	volunteer 90 Pre-Endotoxin Instillation Circulating Neutrophils									True																																																		PRE

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